ANALYSIS OF BACTERIAL LOAD IN DOMESTIC WATER SOURCES IN EKPOMA, EDO STATE, NIGERIA

 
Jemikalajah, Daniel Johnson
Background: Drinking water must be free from harmful microorganisms that can cause serious ill health. Supplies of drinking water may be contaminated with sewage allowed to seep into wells or bore-holes, or faecal matter from man and animals passed into rivers, streams or pools of water.
Objectives: This study was carried out to ascertain the bacteriologic quality of domestic water sources used in Ekpoma, Edo State, Nigeria from February 2016 to June 2017.
Methods: Ten samples each of domestic water sources (sachet water, bore-holes, storage tanks, wells and river) were collected and bacteriological analysis was carried out using the total viable count and multiple tube fermentation technique to determine the most probable number of coliforms/ E. coli.
Results: The highest mean total viable count (TVC) of bacterial load of 4.1 x 106 CFU/ml was obtained for river water followed in descending order by 1.5 x 106 CFU/ml, 7.5 x 105 CFU/ml, 3.2 x 105 CFU/ml and 1.9 x 10 CFU/ml for reservoir well, reservoir tank, bore-hole and sachet water sources respectively on nutrient agar. The highest mean total viable count of bacterial load of 4.1 x 103 CFU/ml was recorded for reservoir tank followed by 1.3 x 103 CFU/ml, 3.7 x 102 CFU/ml, 3.6 x 102 CFU/ml and 0.0 CFU/ml for reservoir well, bore-hole, river water and sachet water sources respectively on macConkey agar. The highest mean of most probable number (MPN) for the presumptive total coliform counts of 140 MPN/100ml was observed for river and the lowest 0.4 MPN/100ml for sachet water. Also, the highest mean of most probable number (MPN) for faecal Escherichia coli counts of 31 MPN/100ml was obtained for reservoir well and the lowest 0.0 MPN/100ml for sachet water.
Conclusion: This study has shown that there is urgent need for an effective, thorough sanitary condition and proper purification given the bacteriological state of these water bodies in order to maintain good quality water.

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PTERYGIUM EXCISION WITH PRIMARY CLOSURE IN SOKOTO, NORTH WEST NIGERI

Mohammed Dantani Adamu

Background: The essence of pterygium surgery is to excise the pterygium and inhibit recurrence. Multiple surgical techniques have evolved over the years to excise the pterygium and inhibit its recurrence.
Objective: To determine the outcome of pterygium excision with primary closure in terms of recurrence.
Methods: A retrospective review of medical records of all patients who had pterygium excision with primary repair during the study period at Usmanu Danfodiyo University Teaching Hospital and Specialist Hospital, Sokoto was undertaken. Data on demographic variables, extent of pterygium and pterygium recurrence after 6 months and postoperative
complications were extracted and analyzed.
Results: There were a total of 32 eyes in 32 patients studied with age range between 26 to 68 years {mean age of 47.2 (SD +12.32)}. There were 15 males and 17 females (M:F = 1:1). Majority of the patients had stage 3 pterygium (65.3%) followed by stage 2 (27.2%) and stage 4 pterygium (7.5%) was the least in frequency P=0.02, CI (2.50-2.95). There were twenty-nine patients (90.6%) with primary pterygia while three (9.4%) had recurrent pterygia. 56.2% of the patients had occupations with possible significant exposure to actinic damage. Recurrence occurred in 12.5% of the patients after 6 months’ follow-up.
Conclusion: Pterygium excision with primary closure appears to be safe and effective. Future comparative case series and randomized controlled studies are recommended

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EFFECT OF SUB-CHRONIC ORAL EXPERIMENTAL EXPOSURE OF MONOSODIUM GLUTAMATE ON BIOMARKERS OF HEPATIC AND RENAL FUNCTION IN MALE WISTAR RATS

 
Hassan Abdulsalam, Jamila A. Atata, Ali Waziri, Mohammed A. Chiroma, Joseph J. Gadzama, Modu B. Monguno, Sani N. Abdulazeez, Israel J. Barde
Background: Monosodium glutamate (MSG) is a food additive that acts as a preservative or as enhancer of palatability and is locally called Ajinomoto or white magi in Nigeria.
Objectives: This study assessed the effect of sub-chronic oral exposure to monosodium glutamate (MSG) on some plasma biomarkers of hepatic and renal function in adult male Wistar rats.
Methods: Sixty-four adult male Wistar rats of average weight 150 to 200 g were randomly assigned into two groups of MSG-treated and non-MSG-treated control rats (n=64). The control rats were orally given distilled water only while the MSG-treated rats were administered 5 g/kg body weight of MSG at concentration of 500 mg/ml daily throughout the period of the experiment that lasted 8 weeks; feed and water were provide to both groups ad libitum. A total of 8 rats, 4 per group were sacrificed weekly for blood sample collection and analysis of hepatic and renal biochemical indices.
Results: the result of biochemical analysis showed significant increase (P ≤ 0.05) in mean of Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Urea and Creatinine activities in the MSG-treated rats.
Conclusion: The increases in the levels of plasma biochemical parameters signal some level of alteration in the normal physiologic function of both liver and kidney.

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Written by Administrator On Sunday, 24 March 2013 17:23
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